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. 2018 May 18;46(15):7924–7937. doi: 10.1093/nar/gky394

Figure 6.

Figure 6.

Helix-destabilizing activities of σNS and NSP2, examined by single-pair FRET (spFRET). (A) Histogram of spFRET efficiency of the dual-labeled RNA stem-loop (10 pM, shown in gray), and in the presence of 25 nM NSP2 (orange). (B) Species-selective correlation analysis of the high-FRET (green autocorrelation function, ACF) and low-FRET (red ACF) populations, and freely diffusing RNA (orange). A typical ACF of a freely diffusing stem-loop is shown in gray (RNA*). Note rightward shift in ACFs of protein-bound stem-loops due to slower diffusion. (C) Histogram of spFRET efficiency of the dual-labeled RNA stem-loop (see panel A), alone (gray) and in the presence of 25 nM σNS (blue). (D) Species-selective correlation analysis of the high-FRET (blue ACF), intermediate FRET (green ACF) and low-FRET (red ACF) populations, and freely diffusing folded RNA (high-FRET, orange). A typical ACF of a freely diffusing stem-loop is shown in gray (RNA*). Only intermediate and low-FRET species are bound to σNS.