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. 2018 May 18;46(15):7924–7937. doi: 10.1093/nar/gky394

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — NSP2 and σNS display different electrostatic contributions to RNA binding. (A and B) Salt-dependence of NSP2 (A) and σNS (B) binding to unstructured 20-mer ssRNA measured by fluorescence anisotropy. (C) Linear correlation between log(K_D) and log([NaCl]) for both NSP2 (black) and σNS (red). Derived mean K_d ± sd (N = 3) values are summarized in Supplementary Table S3. The number of salt bridges contributing to RNA binding is estimated from fitted slopes, corresponding to <2 for NSP2 and 3–4 for σNS.