Skip to main content
. 2018 May 31;46(15):e90. doi: 10.1093/nar/gky433

Figure 5.

Figure 5.

Editing RNA intermediates of retrotransposon Tf1 mutants and restoring transposition with the dCas13a-mediated RNA editing system. (A) Editing efficiency at residues 835A and 1165A in the Tf1-835A and Tf1-1165A mutants, respectively. Residues targeted by the designed crRNA–pRNA are indicated with *. All values are expressed as the mean ± s.e.m. with n = 2. (B) Transposition efficiency of Tf1 mutants (as shown in Figure 5A) with mutant residues targeted by the dCas13a-mediated RNA editing system. Cells were replica plated to YES plates with 5-FOA or 5-FOA+G418. All values are expressed as the mean ± s.e.m. with n = 3.