Table 1.

Editing of endogenous gene transcripts by dCas13a-hADAR2d in S. pombe*

Target gene	Position of target editing residue (A)	a Num of mRNA molecules per cell	bNum of G+C/total	c RNA editing efficiency
tdh1	79	560	23/65	59%, 50%
act1	1566	180	19/65	19%, 12%
mel1	921	0.069	26/65	13%, 10%
ade6	1160	9.8	27/65	10%, 8%
ade6	1003	9.8	24/65	8%, 7%
ade6	622	9.8	28/65	Not detected
erp5	672	6.8	30/65	14%, 12%
mug45	530	0.24	26/65	Not detected
nmt1	648	340	25/65	Not detected

*crRNA–pRNA construct was designed for each target with derived optimal settings, i.e. pRNA with a length of 37 bp, and editing site located in pRNA region 6 bp away from crRNA–pRNA boundary. They were placed in the same plasmid (under rrk1 promoter) as described (Figure 2B).

^aAccording to reference (34,35).

^bWithin 65 bp flanking sequence of editing residue.

^cDetected by Sanger sequencing (‘Materials and Methods’ section and Supplementary Figure S2).