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. 2018 Jun 14;46(15):e93. doi: 10.1093/nar/gky447

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Involvement of miR-196a in HCC tumorigenicity. (A) MiR-196a expression in different HCC cells. (B) Recombinant BVs that expressed scramble sponge (Bac-Scramble), miR-196a sponge (Bac-s196) and pre-miR-196a (Bac-m196). (C–E) miR-196a levels in HepG2 (C), PLC (D) and Huh-7 (E) cells after BV transduction. (F–G) Spheroid formation in PLC (F) and Huh-7 (G) cells after BV transduction. The miR-196a levels in various HCC and normal cells were quantified by TaqMan^® RT-qPCR and normalized to that in human hepatocytes. HCC cells were mock-transduced or transduced with recombinant BVs at MOI 200 and the miR-196a levels were analyzed by RT-qPCR at 1 dpt. The data represent means±SD of triplicated culture experiments.