Figure 3.

Recombinant BVs carrying the synthetic switch for transgene expression regulation. (A) Schematic illustration of recombinant BVs LKE and CirCE. CMV, cytomegalovirus immediate-early promoter. WPRE, woodchuck hepatitis virus posttranscriptional regulatory element. PA, poly A signal. (B) Schematic illustration of transgene expression induction (ON) and repression (OFF) in HCC and normal cells. CirCE transduction of PLC cells (high miR-196a, low miR-126) would turn ON EBFP expression due to the negligible miR-126 levels and high miR-196a levels that could turn OFF L7Ae translation. In contrast, CirCE transduction of HUVEC cells (low miR-196a, high miR-126) would turn ON L7Ae/EYFP expression due to the lack of miR-196a-mediated inhibition and turn OFF EBFP due to the miR-126 and L7Ae binding to the EBFP mRNA.