Figure 7.

Gata4-deleted adult cardiac endothelial cells have dysregulation of cell cycle and angiogenesis-related genes. RNA sequencing analysis of selected genes from sorted Tie2 lineage–traced eGFP⁺ CD31⁺ cells from hearts of adult mice with or without Gata4 deletion mediated by the Tie2^CreERT2 transgene (4 weeks of tamoxifen). Data show fold change in mRNA between Gata4-deleted and wild-type endothelial cells, and are organized to highlight genes relevant to previously defined endothelial gene sets or those known to interact with the transcription factor Gata4. n=2 per group (2 combined digested hearts each). See Methods in the online-only Data Supplement for description of bioinformatics and by DESeq2 analysis to show genes differentially expressed. *P<0.05 vs. WT endothelial cells. Eight of the mRNAs shown were not statistically significant by DESeq2 but are included because they were significantly changed by RT-PCR (see Figure IV in the online-only Data Supplement). ECM indicates extracellular matrix; eGFP, enhanced green fluorescent protein; RT-PCR, reverse transcriptase polymerase chain reaction; and WT, wild type.