The correlation between CD45lowCD271high cell counts and increasing age in samples from female (Fig. 3-A) and male (Fig. 3-B) donors is shown. The results of the 2 methods for the counting of CD45lowCD271high cells (the Attune and LSR II-based methods) and CTP counts using CFU-F assay were compared. For the analysis in female donors (Fig. 3-A), 18, 16, and 15 samples were included, respectively. For the analysis in male donors (Fig. 3-B), 19, 14, and 14 samples were used, respectively. A Spearman r test was used for the correlation analysis.