Figure 2.

Optimization of Lentiviral Gene Transfer in CD4⁺ and CD8⁺ T Cells

(A and B) Transduction efficiency was evaluated on days 5 (D5) and 12 (D12). The transduction efficiency for CD4⁺ (A) and CD8⁺ (B) T lymphocytes are shown, with the percentage of transduced cells in the left-hand panels and the VCN on day 12 in the right-hand panels. The data correspond to three independent experiments and are represented as the mean ± SEM. **** p < 0.0001, calculated using a mixed logistic regression model, compared to PS for transduction percentage; *p < 0.05, calculated with a permutation model.