Figure 3.

Characterization of CD4⁺ and CD8⁺ T Lymphocytes

(A) shows the CD4⁺ and CD8⁺ T cells’ phenotype before culture and on day 5 in all five transduction settings on representative fluorescence-activated cell sorting (FACS) plot. Proportion of naive T cells (Tn), effector memory T cells (Tem), and central memory T cells (Tcm) were analyzed using the CD44 and CD62L markers. (B) The proportions of the different CD4⁺ and CD8⁺ subpopulations in all three experiments are shown after 5 days (left panels) or 12 days (right panels) of culture. No statistically significant difference was observed in the percentages of different T cell subpopulations between different conditions. We analyzed the following subpopulation: CD62L⁺CD44⁻ T cells corresponding to T_N/SCM for naive and stem memory T cell; CD62L⁺CD44⁺ corresponding to T_CM for central memory T cell; and CD62L⁻CD44⁺ corresponding to T_EM for effector memory T cell.