Optimization of Lentiviral Gene Transfer in Sca1+ Cells
Transduction efficiency was evaluated on days 5 and 12, with the proportion of transduced cells in the left-hand panel and the VCN on day 12 in the right-hand panel. The data correspond to three independent experiments and are represented as the mean ± SEM. ****p < 0.0001, calculated using a mixed logistic regression model, compared to PS for transduction percentage; *p > 0.05, calculated with a permutation model.