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Penetration of the targeted nanoconjugates in tumor spheroids. Spheroids of A375/GFP cells were treated with 8PEG-IR700, RGD-8PEG-IR700, and polymer NPs for 4h. After washing, the spheroids were observed with confocal microscopy. Green: GFP fluorescence. Red: IR700/TAMRA fluorescence. Scale bar: 100 μm.
