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. 2018 Sep 5;38(36):7833–7843. doi: 10.1523/JNEUROSCI.1266-18.2018

Figure 4.

Figure 4.

S1PR3 uses distinct G-protein-coupled pathways to activate subsets of somatosensory neurons. a, Percentage of DRG neurons responding to 1 μm S1P after 15 min incubation with either DMSO-Ringer's vehicle (Veh), 100 μm gallein (Gal; Gβγ blocker), 1 μm U-73122 (U73; PLC blocker), or U-73122 + gallein; p < 0.0001 [one-way ANOVA (F(3,28) = 31.46); n = 4–10 wells of ∼50 neurons each per treatment from 2 animals]. Error bars indicate mean responses ± SD. Dunnett's multiple-comparisons p-values are indicated on graph: ***pGal vs. VEH < 0.0001; ***pU73 vs. VEH < 0.0001; ***pGal/U73 vs. VEH < 0.0001. b, From experiments in a, percentage of total neurons sensitive to S1P, AITC, and capsaicin (white, left) or S1P and capsaicin only (gray, right). Error bars indicate mean responses ± SD; p < 0.0001 [one-way ANOVA (F(3,28) = 41.48]. Dunnett's multiple comparisons p-values are represented on graph: ***pGal < 0.0001; ***pU73 < 0.0001; pGal/U73 = 0.98. c, Data displayed in b now plotted as percentage of S1P-responsive neurons that were sensitive to AITC and capsaicin (white) or capsaicin only (gray); p < 0.0001 [one-way ANOVA (F(3,28) = 41.48]. Dunnett's multiple-comparisons p-values are indicated on graph: ***pGal < 0.0001; ***pU73 < 0.0001; pGal/U73 = 0.98.