(A) SND treatment reverses the hydrocortisone-induced suppression of pituitary ACTH and hypothalamic CRF as measured using immunohistochemistry. Images are representative of three tissue slices (magnification, ×40; scale bars, 50 µm). Quantified data of pituitary (B) ACTH and (C) hypothalamic CRF are from triplicate measurements (n=6/group). ##P<0.01, ###P<0.001 vs. blank group; **P<0.01, ***P<0.001 vs. Model group. SND, Sini decoction; ACTH, adrenocortical hormone; CRF, corticotropin-releasing factor.