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Wnt signaling pathway was activated in the CCI model. (A-C) The mRNA levels of Wnt-3a, Frizzled 4 and β-catenin in the spinal dorsal horn of Naive, Sham and treatment group were detected by RT-PCR (n=3). (D and E) Western blotting was performed to detect and quantify the expression of Wnt-3a, Frizzled 4 and β-catenin in Naive, Sham and treatment group (n=3) (*P<0.05). CCI, chronic constriction injury.
