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. 2018 Jul 27;16(4):3233–3240. doi: 10.3892/etm.2018.6533

Figure 6.

Figure 6.

Knockdown of Nrf2 effectively abrogates the protective effect of resveratrol on kidney cells in vitro. (A) HK2 cells were transfected with negative control or siRNA-Nrf2 for 24 h, and the expression of Nrf2 protein was measured by western blot analysis. (B-D) HK2 cells were transfected with negative control or siRNA-Nrf2 for 12 h, and then treated with LPS (10 µg/ml) in the presence or absence of resveratrol for 12 h. RNA was extracted and the mRNA expression of (B) KIM-1 (C) TNF-α and (D) IL-1β was assessed using reverse-transcription quantitative polymerase chain reaction. Values are expressed as the mean ± standard deviation (n=3). *P<0.05 vs. control; #P<0.05 vs. LPS group; $P<0.05 vs. LPS+Resveratol. siRNA, small interfering RNA; TNF, tumor necrosis factor; IL, interleukin; LPS, lipopolysaccharide; Nrf2, nuclear factor-erythroid-2-related factor 2; KIM-1, kidney injury molecule 1.