Figure 2.

Inhibition of recombinant human CYP2E1 enzymes by phillygenin. (a) Chlorzoxazone at a concentration near its Km was incubated with CYP2E1 enzymes and cofactors in the presence of diethyldithiocarbamate (positive control) (0 to 80 μM) or phillygenin (0 to 80 μM). Each point represents the average of duplicate incubations. (b). Lineweaver-Burk plots showing the inhibition of CYP2E1-catalyzed chlorzoxazone 6-hydroxylation by phillygenin (0, 10, 20, and 40 μM) in human recombinant human CYP2E1 enzymes. Each data point represents the average of duplicate experiments.