FIG 2 .

TaxAss validation with tags simulated from full-length Marathonas Reservoir clone libraries. Tags simulated by trimming full-length sequences to the V4 region were classified by TaxAss, and the resulting classifications were compared to “reference” classifications determined by manually aligning the full-length sequences to the FreshTrain. Correct classifications are in green, lost ecosystem-specific classifications are in yellow, and incorrect classifications are in red. (Left) Number of unique sequences in each classification category at fine-resolution taxon levels. (Right) Examples of classifications that fit into each classification category. Tabular results from this and additional amplicon region simulations are available in Table S1.