Figure 3.

Effects of osthole on VEGF and NF-κB signaling in tumor tissues of orthotopic HCC-bearing mice. (A) Tumor tissues were collected following osthole treatment. Levels of VEGF in tumor tissues were measured by ELISA. (B) Protein expression of IκB-α and NF-κB p65 were detected by western blot analysis. Equal loading was confirmed by stripping immunoblots and reprobing for GAPDH. Statistical analysis of IκB-α and NF-κB p65 quantification. Data are represented as the mean ± standard error of the mean. *P<0.05, **P<0.01, compared with the model control group. VEGF, vascular endothelial growth factor; NF-κB, nuclear factor-κB; IκB-α, inhibitor of κB-α.