Figure 1.

Effect of sequential treatment with CLC and BTZ on colorectal cancer cell survival. (A) Cells were treated with BTZ or CLC alone or in combination. Cell viability was measured using an AlamarBlue assay. The levels of fluorescence are presented. n=3. *P<0.01. (B) Cells were sequentially treated with the indicated drug combinations for 24 h. For comparison, cells were treated with 80 µM CLC alone for 24 h. Cell viability was also measured using a Cell Counting kit-8 assay. The absorbance at 450 nm is presented. n=3. ^#P<0.01 compared with CLC 2.5 µM followed by BTZ 20 nM. CLC, celecoxib; BTZ, bortezomib; negative, non-treated cell; DMSO, dimethyl sulfoxide control; RFU, relative fluorescence unit.