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. 2018 Sep 3;20(10):1008–1022. doi: 10.1016/j.neo.2018.08.005

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Inhibition of lysosomes anterograde trafficking decreases AXL-dependent cell invasion. (A) Western blot analysis of AXL in whole cell lysates from SK-GT-4-shControl, SK-GT-4-shARL8B, FLO-1-shControl, or FLO-1-shARL8B cells. (B-E) LAMP1 IF analysis. (B) Representative confocal IF images (60×) of LAMP1 (Green) in SK-GT-4-shControl versus SK-GT-4-shARL8B cells or (D) FLO-1-shControl versus FLO-1-shARL8B cells. Arrows point to cells with peripheral lysosomes, while arrowheads indicate cells with perinuclear lysosomes. (C) Quantification of the percentage of SK-GT-4-shControl versus SK-GT-4-shARL8B cells or (E) FLO-1-shControl versus FLO-1-shARL8B cells with either peripheral or perinuclear lysosomes. Data are represented as mean ± SE. (F-I) Transwell invasion assay. (F) Transwell invasion assay representative images (20×) of SK-GT-4-shControl versus SK-GT-4-shARL8B cells or (H) FLO-1-shControl versus FLO-1-shARL8B cells. (G) Transwell invasion assay quantification as the number of invading SK-GT-4-shControl versus SK-GT-4-shARL8B cells or (I) FLO-1-shControl versus FLO-1-shARL8B cells per microscopic field. Data are represented as median ± SD.