Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 3;20(10):1008–1022. doi: 10.1016/j.neo.2018.08.005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

AXL-induced lactate secretion and cell invasion depends on MCT-1 activity. (A) Western blot analysis of MCT-1 in whole cell lysates from SK-GT-4-shControl, SK-GT-4-shMCT-1, FLO-1-shControl, and FLO-1-shMCT-1 cells. (B) Lactate assay measuring lactate secretion in the CM of SK-GT-4-shControl versus SK-GT-4-shMCT-1 cells or FLO-1-shControl versus FLO-1-shMCT-1 cells. (C-F) Transwell invasion assay. (C) Transwell invasion assay representative images (20×) of SK-GT-4-shControl versus SK-GT-4-shMCT-1 cells or (E) FLO1-shControl versus FLO-1-shMCT-1 cells. (D) Transwell invasion assay quantification as the number of invading SK-GT-4-shControl versus SK-GT-4-shMCT-1 cells or (F) FLO-1-shControl versus FLO-1-shMCT-1 cells. Data are represented as median ± SD. (G-J) Real-time quantitative PCR analysis of MCT-1 and AXL mRNA expression in SK-GT-4-shControl versus SK-GT-4-shAXL (G and H), and in FLO-1-shControl versus FLO-1-shAXL (I and J). Data are represented as mean ± SD. (K-L) Western blot analysis of MCT-1 and AXL protein expression in SK-GT-4-shControl versus SK-GT-4-shAXL (K), and in FLO-1-shControl versus FLO-1-shAXL (L).