Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 3;20(10):1008–1022. doi: 10.1016/j.neo.2018.08.005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Supplementary Figure S2 — AXL expression positively correlates with EMT and cell invasion in EAC cell lines. (A) Western blot of total cell lysates from a panel of six EAC cell lines showing expression of epithelial (E-cadherin) versus mesenchymal (vimentin) markers, and AXL protein expression. (B-G) Real-time quantitative PCR analysis of CDH1 (B), CDH2 (C), SNAI1 (D), SNAI2 (E), TWIST1 (F), and VIM (G) relative mRNA expression in a panel of six EAC cell lines. (H-I) Transwell invasion assay. (H) Transwell invasion assay representative images of OE19 and OE33 epithelial cell lines versus SK-GT-4 and FLO-1 mesenchymal cell lines. (I) Transwell invasion assay quantification of OE19 and OE33 versus SK-GT-4 and FLO-1 cell lines as the number of invading cells per microscopic field. Data are presented as mean ± SD.