Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 3;20(10):1008–1022. doi: 10.1016/j.neo.2018.08.005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Supplementary Figure S4 — Pharmacological inhibition of lysosomes anterograde trafficking impairs AXL-dependent cell invasion in EAC cells. (A-D) LAMP1 IF analysis. (A) Representative confocal IF images (60×) of LAMP1 (green) in SK-GT-4 cells or (C) FLO-1 cells treated with vehicle or niclosamide (0.5 μM) for 48 hours. Arrows point to cells with peripheral lysosomes, while arrowheads indicate cells with perinuclear lysosomes. (B) Quantification of the percentage of SK-GT-4 cells or (D) FLO-1 cells with either peripheral or perinuclear lysosomes relative to their respective total cell number after treatment with vehicle or niclosamide. Data are presented as mean ± SE. (E-J) Transwell invasion assay. (E) Transwell invasion assay representative images (20×) of SK-GT-4 cells or (H) FLO-1 cells treated with vehicle or niclosamide (0.5 μM) for 48 hours. (F) Transwell invasion assay quantification as the number of invading SK-GT-4 cells or (I) FLO-1 cells per microscopic field after treatment with vehicle or niclosamide. Data are presented as median ± SD. (G) Trypan blue-viability assay of SK-GT-4 cells or (J) FLO-1 cells following treatment with vehicle or niclosamide.