Figure 5.

NF-κB1 is a direct target of miR-199a-5p. HO-8910 and ES-2 cells were transfected with the miR-199a-5p mimic or miR-NC. A549 and H460 cells were transfected with the miR-199a-5p mimic or miR-NC. (A) The protein expression of NF-κB1 was determined by western blotting, and GAPDH was used as a loading control. (B) Schematic representation of NF-κB1 3′UTRs indicates a putative miRNA target site. (C) Analysis of the relative luciferase activity of OC cells that were co-transfected with NF-κB1-WT or NF-κB1-MUT and miR-NC or miR-199a-5p mimic. All data (n=10) are presented as the mean ± standard error. **P<0.05 vs. miR-NC and NF-κB1-WT co-transfection group. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR, microRNA; MUT, mutated; NF-κB1, nuclear factor κB1; NC, negative control; WT, wild-type; UTR, untranslated region.