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. 2018 Jul 17;16(4):4223–4228. doi: 10.3892/ol.2018.9163

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — PTEN was a target gene of miR-26a-5p. (A) A conserved binding site of miR-26a-5p in the 3′UTR of PTEN was identified. (B) Dual luciferase reporter assay indicated that miR-26a-5p significantly suppressed the relative luciferase activity of pmirGLO-PTEN-3′UTR as opposed to the activity seen with the control in 293 cells. (C) Western blot analysis also showed that overexpression of miR-26a-5p suppressed the expression of PTEN, while inhibition of miR-26a-5p increased the protein level of PTEN in HT1376 cells (D). RLU: Relative luciferase units. *P<0.05, ***P<0.001 vs. control.