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. 2018 Jul 16;16(4):4343–4352. doi: 10.3892/ol.2018.9149

Figure 1.

Figure 1.

Effect of JFK on cytotoxicity in lung cancer cells. (A) A549, NCI-H1975, NCI-H1650 and NCI-H2228 cells were treated with JFK (0, 0.041, 0.054, 0.081, 0.108, 0.162 and 0.216 mg/ml, respectively) for 48 h, and examined for viability. Data are expressed as the mean ± SD (n=3). (B) A549 cells were exposed to JFK (0.054 mg/ml) for 48 h. Morphological alteration was examined by phase-contrast microscopy. Scale bar, 50 µm. (C) A549 cells were stained with DAPI for detecting nucleus changes using fluorescence microscopy. Scale bar, 25 µm. (D) Cell cycle distribution was evaluated by flow cytometry. (E) Apoptotic cells were determined by flow cytometry after exposure to JFK for 48 h. (F) Ratios of early apoptosis and total apoptosis were analyzed basing on flow cytometric detection. Data are expressed as the mean ± SD (n=3). ***P<0.001. SD, standard deviation; JFK, Jinfukang; PI, propidium iodide; FITC, fluorescein isothiocyanate; IC50, half maximal inhibitory concentration.