Figure 4.

Knockdown of DR4 and Fas attenuates the pro-apoptotic effect of A549 cells induced by JFK. Fas and DR4 in A549 cells were knocked down, and the cells were exposed to JFK (0.054 mg/ml) for 48 h. (A) The cell apoptosis ratio was examined by flow cytometry. (B) Ratios of early apoptosis and total apoptosis were analyzed basing on flow cytometry detection. Data are expressed as the mean ± standard deviation (n=3), *P<0.05, **P<0.01 and ***P<0.001. (C) Fas and DR4 protein expression levels were determined by western blot analysis. DR4, death receptor 4; siRNA, small interfering RNA; JFK, Jinfukang; PI, propidium iodide; FITC, fluorescein isothiocyanate.