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. 2018 Jun 24;17(9):1864–1874. doi: 10.1074/mcp.TIR118.000686

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© 2018 Zhu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 2. — A, comparison of evaporation times for water and DMSO droplets. n = 5 for each condition. B, Evaluation of the capture efficiency of LCM tissue samples using DMSO droplets. A patient-derived xenograft and a rat brain section (12 μm thick) were used as model samples. The replicate numbers were 75, 75, 75, and 27 for tissues having lateral dimensions of 20 μm, 50 μm, 100 μm, and 200 μm, respectively. 200 nL DMSO droplets pre-deposited in nanowells with a diameter of 1.2 mm were used for tissue collection.