Table I. Sequences of primers used for quantitative PCR experiments.
EGL: external granule cell layer. IGL: internal granule cell layer. GL: granule cell layer. ML: molecular layer. PL: Purkinje cell layer. Atoh1: atonal bHLH transcription factor 1. Cadps2: calcium-dependent secretion activator 2. CCK: cholecystokinin. CXCR4: SDF-1 receptor. GAPDH: glyceraldehyde-3-phosphate dehydrogenase, internal standard. Homer1: homer scaffolding protein 1. Neurod1: neurogenic differentiation 1. Oprl1: nociceptin receptor. Pnoc: nociceptin. Stmn1: stathmin 1. Tnr: tenascin R
| Cell layer | Gene name | Primer sequence |
|---|---|---|
| GAPDH | Reverse CAATGTCCACTTTGTCACAAGAGAA | |
| Forward CAGCCTCGTCTCATAGACAAGATG | ||
| Pnoc | Reverse GCGGACGTGAGCTCTGGAT | |
| Forward GCTCCGGGCAGCTTCAA | ||
| Oprl1 | Reverse CAATGTCCACTTTGTCACAAGAGAA | |
| Forward CAGCCTCGTCTCATAGACAAGATG | ||
| EGL | Stmn1 | Reverse CTTTCACCTGAATATCAGAAGATGC |
| Forward CTTTCCTTGCCAGTGGATTG | ||
| Atoh1 | Reverse CCGACAGAGCGTTGATGTAGATC | |
| Forward AACAACGACAAGAAGCTGTCCAA | ||
| ML | Cadps2 | Reverse CAGGTACCCACTATGCTTCATGTG |
| Forward AGGACTCGGACCTAAAGATCAAATT | ||
| Tnr | Reverse ACACCAATGAGCATGTTCTTCAA | |
| Forward AGGACGACATCACGAGATTCCTA | ||
| PL | CCK | Reverse CCAGGCTCTGCAGGTTCTTAA |
| Forward GCACTGCTAGCGCGATACATC | ||
| CXCR4 | Reverse GATGGTGGGCAGGAAGATCCTA | |
| Forward CATGGAACCGATCAGTGTGAGT | ||
| IGL/GL | Homer1 | Reverse GGGGTGATGGTGCTGTTTAT |
| Forward GGAACAGCTATCGGATCATCA | ||
| Neurod1 | Reverse GATGGTTCGTGTTTGAAAGAGAAGT | |
| Forward AAAGCCCCCTAACTGATTGCA | ||