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| Immunostaining characteristics | Sensitivity | Specificity | Accuracy | PPV | NPV |
|---|---|---|---|---|---|
| 1st validation cohort (n = 55) | |||||
| Positive cytoplasmic staining | 1 | 0.982 | 0.991 | 0.982 | 1 |
| Positive nucleic staining | 0.964 | 1 | 0.982 | 1 | 0.965 |
| 2nd validation cohort (n = 60) | |||||
| Positive cytoplasmic staining | 1 | 1 | 1 | 1 | 1 |
| Positive nucleic staining | 1 | 0.8 | 0.9 | 0.833 | 1 |
