. 2018 May 30;17(9):1650–1669. doi: 10.1074/mcp.RA118.000699

Table I. DNA oligonucleotides used for construction of cpcD variants.

Fragment	Primer	Sequence (5′ → 3′)
cpcD upstream frag.	Up-F	`GACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGACGACTTGGAAGTTTGGTTAC`
Overlap: pUC19 & Spec^R	Up-R	`CGCTGCGTTCGGTCAAGAGCTCATTTTGTTAATTACTATTTGAGCTG`
Spec^R cassette	Spec^R-F	`GCTCAAATAGTAATTAACAAAATGAGCTCTTGACCGAACGCAG`
overlap: cpcD-up & cpcD	Spec^R-R	`CAGCAACGAACAAAACTAGGCTATATGGAGTTTGTAGAAACGC`
cpcD mutation frag.	cpcD-F	`TTGCGTTTCTACAAACTCCATATAGCCTAGTTTTGTTCGTTG`
Overlap: Spec^R & point mutation sites	cpcD^Ala-R	`GGTCAAGCTTCTTTGGTGGGCTATGCCAATAC`
	cpcD^Asp-R	`GGTCAAGATTCTTTGGTGGGCTATGATAATAC`
cpcD downstream frag.	cpcD^Ala-F	`TATTGGCATAGCCCACCAAAGAAGCTTGACC`
Overlap: point mutation sites & pUC19	cpcD^Asp-F	`GTATTATCATAGCCCACCAAAGAATCTTGACC`
	Down-R	`GTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCAGTGCGTCGGAGCAATAAGG`