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Specific primers used for qPCR.
| Genes | Nucleotide sequence (5’→3′) | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|
| gtfB | Forward: AGCAAATGCAGCCAATCTACAAAT | 60 | 96 |
| Reverse: ACGAACTTTGCCGTTATTGTCA | |||
| gtfC | Forward: GGTTTAACGTCAAAATTAGCTGTATTAGC | 60 | 91 |
| Reverse: CTCAACCAACCGCCACTGTT | |||
| gtfD | Forward: ACAGCAGACAGCAGCCAAGA | 60 | 94 |
| Reverse: ACTGGGTTTGCTGCGTTTG | |||
| Rec A | Forward: GCGTGCCTTGAAGTTTTATTCTTC | 60 | 75 |
| Reverse: TGTTCCCCGGTTCCTTAAATT |
