Liquid Chromatography
Column:	reversed-phase C-18
Column:	CoreShell column;
Column:	50 mm x 2.1 mm dimensions, 2.6 μm particle size
Column temperature	40 °C
Injection volume:	5 µL
Flow:	0.3 mL/min
Mobile phase:	Solvent A: water containing 0.1% formic acid
	Solvent B: methanol containing 0.1% formic acid
Gradient program:
Time /min	0	1	10	11.1	11.2	12
A:B solvent ratio	99:1	70:30	25:75	1:99	1:99	99:1
Mass Spectrometry
Source:	Dual AJS ESI (positive mode)
Gas and source
Gas Temperature:	300 °C
Drying Gas:	8.0 L/min
Nebulizer:	14 psig
Sheath Gas Temperature:	300 °C
Sheath Gas Flow:	8 L/min
Mass Range:	100 - 1000 m/z
Acquisition Rate:	1 spectrum/s
Acquisition Time:	1000 ms/spectrum
Transient/ spectrum	10014
For targeted MS method
Collision energy (CE):	0 eV
Preferred Mass - Table	734.4685
For MS/MS (typically auto MS/MS mode)
Collision energy (CE):	30 eV
Absolute threshold	3000 counts
Relative threshold	0.01 %
Mass Range:	100 - 100 m/z
Acquisition Rate:	1 spectrum/s
Acquisition Time:	1000 ms/spectrum
Transient/ spectrum	9964
For targeted MS/MS method
Preferred Mass - Table	734.4685