| Reagent | MW | Amount per 1 L | Final |
| HEPES | 238.3 | 11.9 g | 50 mM |
| NaCl | 58.44 | 17.529 g | 300 mM |
| Glycerol | 92.09 | 50 mL | 5% (v/v) |
| Imidazole | 68.1 | 1.3616 g | 20 mM |
| MgCl2 | 203 | 0.20331 g | 1 mM |
| tris(2-carboxyethyl)phosphine | 287 | 2.5 mL 200 mM stock | 0.5 mM |
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