| Reagent | MW | Amount per 1 L | Final |
| HEPES | 238.3 | 7.74 g | 32.5 mM |
| NaCl | 58.44 | 11.686 g | 200 mM |
| Glycerol | 92.09 | 25 mL | 2.5% (v/v) |
| Imidazole | 68.1 | 17.02 g | 250 mM |
| tris(2-carboxyethyl)phosphine | 287 | 0.5 mL 200 mM stock | 0.1 mM |
| DDM (anagrade) | 510.6 | 0.5 g | 0.05% (w/v) |
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