| Component | Volume per reaction (µl) | Final concentration |
|---|---|---|
| Eluted library members | 5 | - |
| Phusion HF buffer (5x) | 10 | 1x |
| Phusion MgCl2 Solution (50 mM) | 2 | 2 mM |
| dNTPs (5 mM) | 2.5 | 250 µM |
| Primer IlluminaPCR1a (10 µM) | 3 | 0.6 µM |
| Primer IlluminaPCR1b (10 µM) | 3 | 0.6 µM |
| Phusion (2 U·µl-1) | 0.25 | 0.5 U per 50 µl |
| Water | up to 50 | - |
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