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. Author manuscript; available in PMC: 2018 Sep 6.
Published in final edited form as: Cancer Res. 2017 Aug 14;77(19):5301–5312. doi: 10.1158/0008-5472.CAN-17-0418

Figure 4. Nuclear FAK and RUNX1 regulate IGFBP3 expression.

Figure 4

(a) Representative anti-FAK western blot of cytoplasmic and nuclear fractions prepared from a series of SCC cells expressing FAK nuclear localization signal (NLS) mutants. (b) (q)RT-PCR analysis of IGFBP3 expression in SCC cells expressing FAK NLS mutants. Tukey’s corrected 1way ANOVA, ****p < 0.0001. Data in all graphs represented as mean +/- s.e.m. n = 3. (c) Predicted transcription factor binding sites in the promoter of Igfbp3. Transcription factors that interact with nuclear FAK in SCC cells are displayed in dark grey. (d) Representative western blot showing Runx1 depletion using shRNA. (e) (q)RT-PCR analysis of IGFBP3 expression in control and Runx1 depleted SCC FAK-wt and SCC FAK-/- cells. Sidak’s corrected 2way ANOVA, ****p < 0.0001. ns = not significant. n = 3.