Figure 5. Nuclear FAK interacts with Runx1.

(a) Representative western blot of anti-FAK immunoprecipitation probed with anti-Runx1 antibody. (b) Representative western blot of anti-Runx1 immunoprecipitation probed with anti-FAK antibody. IgG control (Ctrl). (c) Interaction network analysis of physical or predicted direct binders of Runx1 that interact with FAK in the nucleus of SCC cells (see Supplementary Table 2). Runx1 is shown as a square node. Protein node size is proportional to fold enrichment in nuclear FAK immunoprecipitations. Node color indicates significance of enrichment in nuclear FAK immunoprecipitations. (d) Pathway enrichment analysis of KEGG terms in the nuclear FAK interactome of Runx1 binders (Q < 0.01) (see Supplementary Fig. 5). (e) Representative western blot of anti-phospho-tyrosine (pTyr) immunoprecipitation probed with anti-Runx1 antibody. (f) 2D gel electrophoresis probed with anti-Runx1 antibody. (g) Representative western blot of SCC FAK-wt and FAK-/- nuclear lysates probed with anti-Sin3a antibody. (h) Western blot of anti-Sin3a immunoprecipitation from SCC FAK-wt and FAK-/- nuclear lysates probed with anti-FAK antibody. IgG control (Ctrl) was done using SCC FAK-wt nuclear lysates. (i) Western blot of anti-Runx1 immunoprecipitation from SCC FAK-wt, SCC FAK-/-, and SCC FAK-wt Runx1 shRNA nuclear lysates probed with anti-Sin3a antibody. IgG control (Ctrl) was done using SCC FAK-wt nuclear lysates.