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. Author manuscript; available in PMC: 2019 Jan 6.
Published in final edited form as: ChemMedChem. 2018 Jun 1;13(13):1303–1307. doi: 10.1002/cmdc.201800193

Figure 3.

Figure 3

Binding properties of compound A7/B66/C292 analyzed by Surface Plasmon Resonance (SPR) against HSA immobilized on a BIAcore chip (CM5, 5770 RU). (a) SPR profile of compound R-(A7/B66/C292) at different concentration (5 μM, 1.25 μM, 312 nM, 78.1 nM, 19.5 nM). An overall fitting of the resulting curves (4.7μM, 2.3 μM, 1.2 μM and 582 nM) revealed a koff = 7.4x10-3 Ms-1 and a kon = 2.0x105 s-1M-1, resulting in a Kd = 36.6 nM (b) SPR profile of compound R-(A6/B66/C292) at different concentrations (5 μM, 1.25 μM, 312 nM, 78.1 nM, 19.5 nM). (c) SPR profile of compound R-(A4/B66/C292) at different concentrations (5 μM, 1.25 μM, 312 nM, 78.1 nM, 19.5 nM).