Results of library selections against carbonic anhydrase IX (CAIX). (a) Selection fingerprint. The individual library members are unambiguously identified by their code A (ranging between 1 and 7), B (ranging between 1 and 343) and C (ranging between 1 and 492). The number of sequence counts for each compound is displayed as spheres of different colors, with a cut-off threshold set at 3000 counts. In total, 59,596,519 sequences were read by high-throughput DNA sequencing. (b) Selection fingerprint obtained fixing the code C = 410 (corresponding to acetazolamide) with variable codes A (ranging between 1 and 7) and B (ranging between 1 and 343). This picture shows how the scaffold does not affect the affinity for CAIX.