Table 1.
Allele and protein variants of Tp1–4 observed in the buffalo- and cattle-derived T. parva stabilates
| Sample ID | Stabilate name | Tp1a | Tp2a | Tp3 | Tp4 | ||||
|---|---|---|---|---|---|---|---|---|---|
| Allele TP03_0849b | Protein XP_762973 | Allele TP01_0056 | Protein XP_765583 | Allele TP01_0868 | Protein XP_763892 | Allele TP03_0210 | Protein XP_763228 | ||
| Buffalo-derived T. parva | |||||||||
| BD1 | Mara 3 | 16 | 15 | 8 | 8 | 6c | 4 | 2 | 1 |
| BD2 | Mara 4 | 1 | 1 | 9 | 9 | 10 | 9 | – | – |
| BD3 | Mara 18 | 17 | 16 | 10 | 10 | 7 | 6 | 3 | 4 |
| BD4 | Mara 30 | 1 | 1 | 11 | 11 | 6 | 4 | 3 | 4 |
| BD5 | Mara 32 | 1 | 1 | 12 | 12 | 6 | 4 | 9 | 5 |
| BD6 | Mara 42 | 18 | 17 | 13 | 13 | 8 | 9 | 8 | 6 |
| BD7 | Mara 6998 | 1 | 1 | 14 | 14 | 3 | 8 | 12 | 3 |
| BD8 | Mara 6999 | 19 | 18 | 7 | 7 | 2 | 5 | 5 | 1 |
| BD9 | Mara 7001 | 20 | 14 | 15 | 15 | 4 | 2 | 3 | 4 |
| BD10 | Mara 7546 | 21 | 19 | 16 | 16 | 11 | 9 | 5 | 1 |
| BD14 | Buffalo 7014 | 1 | 1 | 20 | 1 | 5 | 3 | – | – |
| BD15 | Buffalo 7698 | 14 | 12 | 21 | 20 | 10 | 9 | 13 | 3 |
| BD16 | Buffalo 7344 | 24 | 21 | 7 | 7 | 5 | 3 | 3 | 4 |
| BD17 | Mara 41 | – | – | – | – | 5* | 3* | 11d | 1 |
| BD18 | Buffalo 4740 | 1 | 1 | 44 | 43 | 1* | 1* | 13 | 3 |
| BD19 | Buffalo 5012 | 1 | 1 | 1 | 1 | 13*e | 9* | 1 | 1 |
| BD20 | Buffalo 5489 | 36 | 13 | 48 | 47 | 10* | 9* | 13 | 3 |
| BD21 | Buffalo 6666 | 40 | 36 | 48 | 47 | 3* | 8* | 2 | 1 |
| BD22 | Buffalo 6807 | 13 | 11 | – | – | – | – | 4.1f | 1.1 |
| BD23 | Buffalo 6818 | 13 | 11 | – | – | 10* | 9* | 4 | 1 |
| BD24 | Buffalo 6819 | 37 | 35 | 45 | 42 | 10* | 9* | 10 | 1 |
| BD25 | 6998 cl. 11 | 38 | 37 | 49 | 45 | 3 | 8 | 12 | 3 |
| BD26 | Buffalo 7065 | – | – | – | – | – | – | 9.1 | 5 |
| Cattle-derived T. parva | |||||||||
| CD16 | Tc867 BT 106 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| CD17 | Tc841 IL 57 | 2 | 2 | 47 | 46 | 9 | 7 | 7 | 3 |
| CD28 | Tc120F344 | 39 | 17 | 46 | 44 | 12 | 9 | 6 | 2 |
We have previously published (Pelle et al. 2011) the Tp1 and Tp2 results for samples BD1-BD16. They are included here in italics for completeness.
The letters-numbers in this row indicate the reference sequence in the NCBI Genbank.
Newly identified alleles and protein variants are in boldface.
Although we were unable to determine if the sequence is novel due to four unknown (mixed) nucleotides, the sequence is considered novel due to unique nucleotides in other sequence regions.
Unique, validated alleles or protein variants obtained from partial sequences which are their own variant.
A variant with 0.1 after the variant number indicates a sequence which is identical to the parent variant but has 1–4 unresolved nucleotides which prevents its being regarded as completely identical to the parent allele (e.g. 4 if labelled 4.1, as in BD22, Tp4).
partial sequence as defined by only having either the forward or reverse sequence of the allele available for analysis (ie: no complete consensus sequence).