Table 5.
Comparative sequence information on TpAg1–10 observed in the buffalo- and cattle-derived theileria stabilates
| Antigen | Primers 5’ – 3’ Forward Reverse |
Predicted length of amplicona (%) | Lengths of readable sequence | Total no. sequences obtained | Total no. SNP locationsb | Total no. allelesc,d | Total no. non-synonymous SNP locations | Total no. protein variantsc,d | Total no. epitope variants |
|---|---|---|---|---|---|---|---|---|---|
| Tp1 | ATGGCCACTTCAATTGCATTTGCC GAAGCTCATAAATATTTCATT |
387 (22) | 363–399 | 11 | 19 | 8 | 16 | 8 | 2 |
| Tp2 | ATGAAATTGGCCGCCAGATTA GAAGCCTCCGGCACTTCATAG |
483 (47) | 456–480 | 9 | 220 | 7 | 92 | 7 | See Table 2 |
| Tp3 | ATGAAATTAAATACTATCGCAATAG ACCTTCACTAAGAGTCCAGACGATAAT |
735 (89) | 686–726 | 24 | 26 | 13 | 12 | 9 | n/a |
| Tp4 | CCAATTGGTGATTTAGCAACA CTCCGTAAATCATGTTAATGGAGT |
692 (27) | 386–422 | 24 | 36 | 13 | 5 | 6 | 1 |
| Tp5 | ATGAGGAAGCGAGTTTGGG CGATATCGATGATTTATAA |
238 (31) | 200–238 | 16 | 26 | 6 | 3 | 3 | 1 |
| Tp6 | ATGGCTCAGATTCCTGTTG CTCTTACTCTCAACTGATAAATAA |
1005 (92) | 569–833 | 23 | 23 | 13 | 1 | 2 | n/a |
| Tp7 | ATGGAGGCACTGCAAGCAGGC GTCACTGACGAGGAGGAGAAAAAG |
417 (14) | 299–332 | 25 | 33 | 9 | 4 | 3 | 1 |
| Tp8 | ATGCTTGGAAATCATGTCATGGGA CTCCTTCGCAGTGTACGCAGTTTAA | 1275 (81) | 931–1109 | 22 | 19 | 12 | 6 | 7 | 1 |
| Tp9 | ATGAATGTTCTAACTACTGG CGTAATAAACCATGGACAAAACAATAA |
958 (95) | 326–1057 | 37 | >100 | 28 | >100 | 27 | 12 |
| Tp10 | GACTACGCTCTTCTTACCAC CCATCCAGGAGTGTAATGCC |
916 (89) | 505–824 | 22 | 24 | 13 | 7 | 6 | n/a |
a
The sequence length of the predicted amplicon from the reference sequence, excluding primers, is shown. The figures in brackets represent the length of the amplicon as a percentage of the length of the coding sequence from the reference gene, including introns.
b
SNPs within introns are not included.
c
Alleles or protein variants which have ambiguous residues (labelled X.1 in Table 1) are not included, unless they are unique, validated variants.
d
Unique, validated alleles or protein variants obtained from partial sequences are included if the constitute a variant.