Figure 1. DU145 cells that survive docetaxel treatment show increased expression of stem cell markers: α2 integrin and CD44.

(A) The representative FACS plots show increased surface expression levels of α2 integrin and CD44 on cells that have been on collagen I or on fibronectin (5 μg/cm²) coated plates and treated with 50 nM Docetaxel for 24 hours. (B) The quantification of α2 integrin and CD44 cell surface expression based on GeoMean values. Data as mean ± SEM (n=3). ^* = P < 0.05; ^** = P < 0.01; ns. = not significant. (C) The FACS plot illustrates the sorting of DU145WT cells into DU145WT (α2^High) and DU145WT (α2^Low) subpopulations based α2 integrin expression level on cells. (D) Representative FACS plots for α2 integrin, CD44 and Trop2 expression on DU145WT (α2^High) and DU145WT (α2^Low) subpopulations analyzed in the 5th passages after sorting. (E) Quantification of the proliferation of DU145WT (α2^High) subpopulation compared to DU145WT (α2^Low) subpopulation on collagen I, fibronectin and poly-l-lysine measured with the WST-8 assay. Data are mean absorbance values (OD) at 490 nm (n=3). ^* = P < 0.05.