Figure 3. α2 integrin positive DU145 cells are more invasive than α2 negative cells.

(A) Representative microscopic images (10 x) of DU145WT, DU145KO+α2 and DU145KO+vector cells that have invaded through matrigel in the transwell invasion assay. Below, the quantification of the invaded cells. Mean ± SEM (n=5). ^* = P < 0.05. One way ANOVA and Tukey HSD post hoc test. (B) Representative images of DU145KO+α2 and DU145KO+vector cells in migration assay. Cells were allowed to migrate out of spheroids on the collagen I coated surface for 96 hours. The quantitation of cell migration as the area covered by DU145KO+α2 and DU145KO+vector cells. Data are mean ± SEM (n=4). ^* = P < 0.05; ^** = P < 0.01. (C) Representative images of DU145KO+α2 and DU145KO+vector cells in invasion assay. Cells were allowed to invade out of spheroids into the collagen I gel for 96 hours. The quantitation of cell invasion as the area covered with DU145KO+α2 and DU145KO+vector cells. Data are mean ± SEM (n=4). ^* = P < 0.05.