Fig. 6.

Pressure-dependent NMR of pp32 L60A. (A) HSQC spectra of pp32 L60A at multiple pressures from 1 bar (red) to 2 kbar (black) as indicated. Boxed and labeled residues exhibited pressure-dependent double peaks in slow exchange. (B) Double peaks in slow exchange at 600 bar for residues 114, 141, 142, and 145, as indicated. (C) Pressure dependence of the intensity of the two peaks, folded state (filled circles) and intermediate state (open circles) for residue 142. (D) Residues exhibiting double peaks in slow exchange are shown as black spheres on the pp32 structure.