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. 2018 Aug 13;115(35):8805–8810. doi: 10.1073/pnas.1722594115

Fig. 3.

Fig. 3.

Effects of cardiac vegfaa overexpression in the absence of injury. (A–D) Tiled images of cmlc2:CreER;βact2:BS-vegfaa;fli1a:EGFP hearts at 6 wpf and 8 wpf after treatment with vehicle or tamoxifen at 30 dpf. (E and F) Tiled images of adult cmlc2:CreER;βact2:BS-vegfaa;fli1a:EGFP hearts 30 dpi with vehicle or tamoxifen. Sections are immunostained for Tnnt. Boxes correspond to the region magnified in adjacent panels. Blue lines approximate the CM primordial layer and blue arrows denote ectopic vasculature. (G–I) Maximum thickness of the Tnnt+ compact layer at 6 wpf after treating 30-dpf fish with vehicle (n = 11) or tamoxifen (n = 6); at 8 wpf after treating 30-dpf fish with vehicle (n = 11) or tamoxifen (n = 9); and at 30 dpi after treating adult fish with vehicle (n = 5) or tamoxifen (n = 6). Error bars indicate SD (**P < 0.01 and ***P < 0.001, Student’s t test). (Scale bars: A–F, Left, 100 μm; A–F, Right, 25 μm.)