Fig. 5.

Dependence of vegfaa-induced CM hyperplasia on Notch signaling. (A–D) In situ hybridization for notch1b and deltaC on cardiac sections from cmlc2:CreER;βact2:BS-vegfaa animals 14 dpi with vehicle or tamoxifen. Violet staining indicates expression. (E–H) Maximum-intensity projections of 7 dpf hearts from cmlc2:H2A-EGFP;βact2:BS-vegfaa fish with or without an additional cmlc2:CreER transgene. Animals were first treated with 4-HT, followed by DMSO (n = 9 for Cre⁻ animals and n = 10 for Cre⁺ animals) or DAPT (n = 8 for Cre⁻ animals and n = 9 for Cre⁺ animals). (I) Number of CMs at 7 dpf for treatment groups shown in E–H. Error bars indicate SD (*P < 0.05, **P < 0.01, and ***P < 0.001, Student’s t test). NS, not significant. [Scale bars: A and E, 100 μm (also apply to B–D and F–H, respectively).]