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. 2018 Sep 6;13(9):e0203048. doi: 10.1371/journal.pone.0203048

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© 2018 Agostini et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Impact of medium additives A, B, C and D on proliferation rate of ASC. (A) Cell growth test based on luminescence generated by living and proliferating cells. Results show that in presence of 5% medium additive A (recalcified PRP) and B (recalcified PPP) cell proliferation rate is higher, when compared to additives C (freeze and thaw, plasma depleted platelets) and D (freeze and thaw, PRP). (B) Mean values of major-to-minor axis ratios and representative images of ASC expanded in presence of the different additives. ASC expanded in presence of medium additives A and B were more elongated than ASC expanded in C and D. ASC expanded with additive A were more elongated than ASC cultured with additive B. Values of luminescence and of major-to-minor axis ratios obtained from cells expanded in presence of 10% FBS were shown as control condition. Considering such results, ASC expanded in presence of medium additive A showed higher proliferation rate when compared to additive B. When compared to medium additives A and B, ancillary products C and D weakly stimulated cell proliferation. ANOVA for independent samples was performed as statistical analysis (p<0.001) and Tukey’s honestly different significance with Bonferroni’s correction was chosen as post hoc test to compare the effects of the different medium additives.*, p<0.05 vs C and D; **, p<0.01 vs B, C and D; ^§, p<0.05 vs C and D; NS vs D.