Fig 4. Measurement of strains based on changes in the diameter of wells.

(A) Photographs of an identical well before (Unstretch) and after (Stretch) 10% uniaxial stretch. Black arrow represents the direction of stretch. Scale bar, 2 mm. (B) Map of transverse strains at each well position. (C) Map of longitudinal strains at each well position. (D) Transverse strains at different regions of the plate. (E) Longitudinal strains at different regions of the plate. Representative data from N = 2 independent experiments and analyses. Boxes represent the 25th and 75th percentiles and the median. Open squares indicate means. Whiskers extend from the ends of the box to the most remote points excluding outliers. †, P < 0.01 by Welch’s test assuming unequal variance with rejection of outliers; *, P < 0.001 by Student’s t-test.